Identification of Resistance Sources against Bacterial Pustule Disease Caused by Xanthomonas axonopodis pv. glycines in Soybean using Excised Leaf Technique

Bacterial pustule of soybean is a foliar disease caused by Xanthomonas axonopodis pv. glycines. The organism produces pustules which causes premature defoliation and ultimately results in significant reduction seed yield quality. Use resistant cultivars can be cost effective eco-friendly approach to address the issue. This study was aimed at development rapid reliable in-vitro screening method genotypes for identification resistance sources bacterial disease. One hundred thirty-six including released varieties, indigenous exotic germplasm were screened excised leaf technique their reaction An improved developed. Rooting survival studies indicated that leaves from 25 days old plants could survive up 30 room temperature plain water. Inoculation with suspension 107 109 colony forming units (cfu/ml) 27±1°C under photoperiod 12h/day light showed initiation chlorotic lesions 48 hours after inoculation. Based on latent period appearance lesions, classified as susceptible, moderately resistant. Four (TS-3, P-4-2, Hara Soya Himso 1685) while, seventeen (AMS MB5-19, Bhatt, DSb-12, JS-335, JS93-05, MACS- 1188, MAUS- 71, PK-262, PS- 1241, Pusa-5, RKS- 18, SL-688, SL-744, SL-958, SL97-52, SL-979, T99-76) rest susceptible